Instructions: This checklist is meant to serve as a general guideline for our client facilities as to the level of your skills within your nursing specialty. Please use the scale below to describe your experience/expertise in each area listed below.
| Orientation and Lab Safety | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Review the laboratory’s fire safety plan. |
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| Locate Personal Protective equipment and MSDS. |
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| Understand Universal Precautions for microbiology. |
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| Labeling & Specimen ID | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Label specimens according to institutional policies. |
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| Specimen set up & incubation | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Select proper media for specimens including plated media and broth media. |
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| Understand specimen collection & rejection criteria. |
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| Incubate specimens properly. |
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| Inoculation | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Demonstrate plate streaking for isolation & quantitative streaking for urines. |
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| Quality Control | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Perform quality control procedures in accordance with institutional policies for new media, reagents, and stock culture organisms. |
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| Understand documentation & actions taken when results are not in acceptable limits. |
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| Gram Staining | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Practice performing Gram stains until proficient. |
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| Evaluate grams stains, including sputum samples, wounds, genital samples, and positive blood cultures until proficient. |
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| Evaluation of primary cultures | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Evaluate cultures to recognize what is normal flora and what is significant. |
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| Evaluate throat cultures & select next course of action. |
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| Evaluate urine cultures to decide when susceptibility testing is warranted. |
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| Evaluate vaginal cultures to recognize what is normal flora and what is significant. |
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| Evaluate stool cultures to recognize what is normal and what to process further. |
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| Evaluate body fluid cultures for pathogens. |
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| Evaluate wound cultures to recognize what is significant -select next course of action. |
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| Evaluate respiratory cultures, including sputum cultures. Recognize normal resp. flora & significant pathogens. |
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| Blood Culture processing | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Demonstrate procedure for processing positive blood cultures including subcultures, Gram stains, and proper reporting of results. |
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| Primary ID of organisms | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Recognize Streptococcus species & perform tests to classify them according to the Lancefield classification scheme. |
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| Recognize & perform tests to identify Staphylococcus species. |
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| Identify coagulase negative Staphylococcus species. |
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| Recognize and identify Haemophilus species. |
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| Recognize and identify Neisseria species. |
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| Recognize and perform biochemical tests to ID GNBs, including lactose fermenters, nonfermenters, and other miscellaneous GNBs. |
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| Recognize and identify Campylobacter species. |
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| Interpretation and Acceptance of results | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Discuss recording, reporting, and documenting results |
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| Discuss which organisms are reportable to the State Health Department. |
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| Serological testing | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Perform Rapid test for Group A Streptococcus, include proper labeling, QC, and reporting. |
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| Affective Objectives - demonstrates honesty by: | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Maintaining strict patient confidentiality |
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| Accepting control values only when within acceptable limits. |
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| Performing and documenting daily & weekly maintenance procedures, preventative maintenance, temperature checks, etc. |
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| Completing all procedures in adherence to laboratory SOPs, taking no shortcuts or unauthorized modifications of procedure. |
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| Personal Interactive Skills - demonstrates proper professional behavior by: | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Working with co-workers in a positive manner, promoting productive workflow. |
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| Refraining from making statements or actions that represent sexual, ethnic, racial, or homophobic harassment. |
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| Willingly and consistently using appropriate personal safety devices when handling caustic, infectious, or hazardous materials. |
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| Completing all required tasks and remaining in the work area when scheduled. |
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| Being punctual whenever scheduled. |
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| Adhering to current dress and appearance in the laboratory setting. |
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| Cleaning the work area when leaving the laboratory, returning supplies to appropriate storage location, & disinfecting all work areas used by the student. |
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| Professional Responsibility - demonstrates appropriate professional affective behavior by: | Rating Stars (Click) | 1 | 2 | 3 | 4 | ||
| Correctly reporting all patient test values, as well as recognizing and correctly reporting all patient critical test values. |
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| Resolving discrepancies in specimen labeling, handling, or collection before reporting patient results. |
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